Journal: eLife

Article Title: Sloppy morphological tuning in identified neurons of the crustacean stomatogastric ganglion

doi: 10.7554/eLife.22352

Figure Lengend Snippet: Neurons were identified physiologically and then filled with Lucifer yellow. Following fixation and immunohistochemical amplification with a rabbit anti-Lucifer yellow primary antibody and an Alexa Fluor 488-tagged secondary antibody, each dye-fill was imaged at 63x magnification as a series of z-stacks that tiled the neuronal structure across the x-y plane (z-stacks contained slices at increments of ~0.5 µm). These z-stacks were stitched together with custom software written in MATLAB. The image stack of each full three-dimensional neuronal structure was then manually traced (image shown is a screenshot from the KNOSSOS platform). The three-dimensional skeletal structures were then converted to hoc files that could be analyzed with a custom quantitative morphology toolbox composed in Python. All conversion and analytical scripts are freely available on the Marder Lab Github repository. DOI: http://dx.doi.org/10.7554/eLife.22352.024

Article Snippet: These tile stacks were aligned and stitched with a GUI-based MATLAB tool ( ) available at https://github.com/marderlab Confocal_Stitching.

Techniques: Immunohistochemical staining, Amplification, Software